Abstract: 【Objective】To explore the effects of three different culture modes on the nutritional quality of tilapia muscle, including in-pond raceway system aquaculture(IPRS group), paddy field aquaculture(paddy field group) and pond aquaculture(pond group), so as to provide reference for improving the quality and efficiency of tilapia culture.【Method】The GIFT(Oreochromis niloticus) in IPRS group, paddy field group and pond group were selected with body weights at 595±88, 625±75 and 644±21 g, respectively.The nutritional value of GIFT muscle was evaluated by analyzing and comparing the nutritional components of muscle with each gram of nitrogen amino acid score model, complete protein score model and texture characteristic parameters.【Result】In terms of conventional nutrients, the water content and crude ash content had no significant difference among the three groups(P> 0.05), the crude fat content of GIFT muscle in IPRS group was significantly higher than that in pond group and paddy field group(P< 0.05, the same below), and the crude protein content of GIFT muscle in paddy field group was significantly higher than that in IPRS group and paddy field group.In terms of amino acid composition, aspartic acid(Asp), glutamic acid(Glu), leucine(Leu) and lysine(Lys) contents were the highest.The total essential amino acid(ΣEAA) of GIFT muscle in paddy field group was significantly higher than that in pond group and IPRS group.The EAA/total amino acid(ΣTAA) ranged from 0.39 to 0.41, and ΣEAA/total non-essential amino acid(ΣNEAA) ranged from 0.65 to 0.68 in the three groups.In the evaluation of EAA, the total amount of EAA(2689 mg/g N) and the index of essential amino acid(EAAI, 87.96) in GIFT muscle of IPRS group were the highest, followed by pond group and rice field group.In terms of muscle texture characteristics, the hardness of GIFT muscle in IPRS group was significantly lower than that in paddy field group and pond group, and the stickiness was significantly higher than that in paddy field group and pond group.【Conclusion】The comprehensive evaluation of GIFT meat quality under the pond engineering recirculating water culture mode is the best, and the culture effect is good, which is consistent with the requirements of energy conservation, emission reduction, quality improvement and efficiency, so it can be vigorously promoted in suitable areas.