Optimization of ultrasonic assisted extraction of polyphenol from Euryale ferox seed shells and its antimicrobial activity against Staphylococcus aureus
-
摘要: 【目的】优化芡种壳多酚提取工艺，并分析其对金黄色葡萄球菌的抑制作用和抑菌稳定性，为芡种壳的资源化利用提供参考依据。【方法】采用超声辅助法提取芡种壳多酚，以多酚提取率（Y）为评价指标，对超声时间（X1）、超声温度（X2）和料液比（X3）3个因素进行单因素和响应面试验，确定最佳提取工艺，初步纯化；采用琼脂扩散法研究芡种壳多酚对金黄色葡萄球菌的抑制活性，并测定最小抑菌浓度（MIC），同时考察温度、pH及金属离子浓度对芡种壳多酚稳定性的影响。【结果】3个因素对芡种壳多酚提取率影响的大小为：超声温度>料液比>超声时间，料液比及超声温度与料液比的交互作用对芡种壳多酚提取率有显著影响（P< 0.05），超声温度对多酚提取率有极显著影响（P< 0.01）。得到回归方程Y=14.57+0.064X1+0.26X2+0.11X3+0.079X1X2-0.018X1X3-0.18X2X3-0.11X12-0.54X22-0.19X32；最佳提取工艺为：超声时间39 min、超声温度72 ℃、料液比1：41，在此条件下，芡种壳多酚提取率为14.479%，与预测值（14.630%）接近；芡种壳多酚对金黄色葡萄球菌具有明显抑制作用，MIC为0.1625 mg/mL；温度及Ca2+、Mg2+、K+、Na+4种金属离子对芡种壳多酚抑制金黄色葡萄球菌活性不具有显著影响（P> 0.05），而随着pH的增加，芡种壳多酚抑菌活性受到抑制。【结论】采用优化的超声辅助法可有效提取芡种壳中多酚物质，提取到的多酚对金黄色葡萄球菌的生长具有良好且较稳定的抑制效果。Abstract: 【Objective】The extraction technology of polyphenols from Euryale ferox seed shell was optimized, and its inhibition and stability to Staphylococcus aureus were analyzed to provide basis for resource utilization of E.ferox seed shell.【Method】The ultrasonic assisted extraction method was used to extract the polyphenols from E.ferox seed shell.The single factor experiment and response surface experiment with extraction yield of polyphenols(Y) as the evaluation index, and the ultrasound time(X1), ultrasound temperature(X2) and soild-liquid ratio(X3) as three factors were carried out to determine the best extraction process, then the extract was preliminarily purified by macroporous resin.The inhibitory activity and the minimum inhibitory concentration(MIC) of E.ferox seed shell polyphenols on S.aureus was studied by agar diffusion method.The effects of temperature, pH and metal ion concentration on the stability of polyphenols were also investigated.【Result】The effects of three factors on yield of polyphenols from E.ferox seed shell were:ultrasound temperature> solid-liquid ratio> ultrasound time, the results showed that the solid-liquid ratio, and the interaction between ultrasound temperature and solid-liquid ratio had significant effects on yield of polyphenols(P< 0.05), while the ultrasound temperature had extremely significant effects(P< 0.01) on the extraction rate of polyphenols from E.ferox seed shell.The regression equation was obtained:Y=14.57+0.064X1+0.26X2+0.11X3+0.079X1X2-0.018X1X3-0.18X2X3-0.11X12-0.54X22-0.19X32.The optimum extraction process was:ultrasound time was 39 min, ultrasound temperature was 72℃, solidliquid ratio was 1:41.Under these conditions, the extraction rate was 14.479%, which was close to the predicted value(14.630%). E.ferox seed shell polyphenol had certain inhibitory effect on S.aureus, the MIC was 0.1625 mg/mL.The temperature and Ca2+, Mg2+, K+ and Na+ metal ions had no significant effect on the inhibition of S.aureus reproductive activity by E.ferox seed shell polyphenols(P> 0.05), but with the increase of pH, the bacteriostatic activity of E.ferox seed shell polyphenols was inhibited.【Conclusion】The polyphenols in the shell of E.ferox can be effectively extracted by the optimized ultrasonic assisted method, and the polyphenols has a good and stable inhibitory effect on the growth of S.aureus.
-
-
陈德义, 朱西平, 张弛, 袁怀波.2015.芡实壳矿质元素及其油状物成分测定[J].食品科技, 40(1):322-326.[Chen D Y, Zhu X P, Zhang C, Yuan H B.2015.Analysis of mineral elements and volatile chemical constituents in seed coat of Euryale ferox[J].Food Science and Technology, 40(1):322-326.] 韩雯雯.2012.番泻叶抑菌成分的提取分离与抑菌作用研究[D].呼和浩特:内蒙古农业大学.[Han W W.2012.Extraction and separation of bacteriostatic compositions from senna and study on its antimicrobial activity[D].Huhhot:Inner Mongolia Agricultural University.] 黎卫.2015.芡实蛋白组分的结构表征及其理化性质研究[D].无锡:江南大学.[Li W.2015.Structure characterization and physicochemical properties of Euryale ferox seed protein fractions[D].Wuxi:Jiangnan University.] 李成良.2011.芡实醇提物的抗氧化、抑菌作用及PPO性质研究[D].扬州:扬州大学.[Li C L.2011.Study on antioxidation, bacteriostasis and PPO properties of alcohol extract from Euryale ferox[D].Yangzhou:Yangzhou University.] 刘志国, 赵文亚.2012.芡实多糖对小鼠抗运功性疲劳作用的研究[J].中国农学通报, 28(21):269-271.[Liu Z G, Zhao W Y.2012.The research on gorgon polysaccharide of function on anti-fatigue in rat[J].Chinese Agricultural Science Bulletin, 28(21):269-271.] 王晶.2011.芡实淀粉性质的研究[D].长春:吉林农业大学.[Wang J.2011.Study on the properties of Euryale ferox starch[D].Changchun:Jilin Agricultural University.] 王娜, 褚路路, 徐梦, 乔高翔, 郑国栋, 杨丽聪.2019.没食子酸修饰纳米硒的制备表征及其抑菌活性研究[J].食品科技, 44(1):302-307.[Wang N, Chu L L, Xu M, Qiao G X, Zheng G D, Yang L C.2019.Synthesis and antibacterial activity evaluation of gallic acid modified selenium nanoparticle[J].Food Science and Technology, 44(1):302-307.] 张汆, 陈志宏, 何晓伟, 于士军, 柏钰.2015.芡种壳提取物抗氧化、抑菌活性及其组分分析[J].食品工业科技, 36(22):151-155.[Zhang C, Chen Z H, He X W, Yu S J, Bai Y.2015.Antioxidative and antibacterial activities of Euryale ferox Salisb.shell extracts and its compositions analysis[J].Science and Technology of Food Industry, 36(22):151-155.] 张名位, 池建伟, 孙玲, 徐志宏, 李健雄.1999.潮州芡实的营养学评价[J].广东农业科学, (2):27-29.[Zhang M W, Chi J W, Sun L, Xu Z H, Li J X.1999.Nutritional evaluation of Euryale ferox in Chaozhou[J].Guangdong Agricultural Science, (2):27-29.] 赵毅.2015.甘蔗多酚分离鉴定及生物活性研究[D].广州:华南理工大学.[Zhao Y.2015.Study on isolation and identification of sugarcan polyphenols and their biological activity[D].Guangzhou:South China University of technology.] Baek S H, Nam I J, Kwak H S, Kim K C, Lee S H.2015.Cellular anti-melanogenic effects of a Euryale ferox seed extract ethyl acetate fraction via the lysosomal degradation machinery[J].International Journal of Molecular Sciences, 16(5):9217-9235.
Das S, Der P, Raychaudhuri U, Maulik N, Das D K.2006.The effect of Euryale ferox(Makhana), an Herb of aquatic origin, on myocardial ischemic reperfusion injury[J].Molecular & Cellular Biochemistry, 289(1-2):55-63.
Huang Z H, Huang X Y, Wang Q, Jiang R Z, Sun G D, Xu Y M, Wu Q N.2018.Extract of Euryale ferox Salisb exerts antidepressant effects and regulates autophagy through the adenosine monophosphate-activated protein kinase-UNC-51-like kinase 1 pathway[J].International Union of Biochemistry and Molecular Biology Life, 70(4):300-309.
Li G H, Wang X, Xu Y F, Zhang B G, Xia X D.2014.Antimicrobial effect and mode of action of chlorogenic acid on Staphylococcus aureus[J].European Food Research & Technology, 238(4):589-596.
Liu Y, Wei S L, Liao M C.2013.Optimization of ultrasonic extraction of phenolic compounds from Euryale ferox seed shells using response surface methodology[J].Industrial Crops and Products, 49:837-843.
Najdenski H M, Gigova L, Iliev I, Pilarski P, Lukavský J, Tsvetkova I, Ninova M S, Kussovski V.2013.Antibacterial and antifungal activities of selected microalgae and cyanobacteria[J].International Journal of Food Science & Technology, 48(7):1533-1540.
Nam G H, Jo K J, Park Y S, Kawk H W, Kim S Y, Kim Y M.2019.In vitro and in vivo induction of p53-dependent apoptosis by extract of Euryale ferox Salisb in A549 human caucasian lung carcinoma cancer cells is mediated through Akt signaling pathway[J].Frontiers in Oncology, 9:1-13.
Singleton V L, Orthofer R, Lamuela-Raventós R M.1999.Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent[J].Methods in Enzymology, 299C(1):152-178.
Song C W, Wang S M, Zhou L L, Hou F F, Wang K J, Han Q B, Li N, Cheng Y X.2011.Isolation and identification of compounds responsible for antioxidant capacity of Euryale ferox seeds[J].Journal of Agricultural & Food Chemistry, 59(4):1199-1204.
Wu C Y, Wang H, He X X, Wu D W, Yue W, Wu Q N.2017.Hypoglycemic and antioxidant effects of polysaccharides from the petioles and pedicels of Euryale ferox Salisb.on alloxan-induced hyperglycemic mice[J].Food & Function, 8(10).doi: 10.1039/C7FO01035D.
Wu J Y, Guan R F, Cao G Z, Liu Z F, Wang Z, Shen H T, Xia Q L.2018.Antioxidant and antimicrobial effects of catechin liposomes on Chinese dried pork[J].Journal of Food Protection, 81(5):827-834.
Xiong L G, Chen Y J, Tong J W, Huang J A, Li J, Gong Y S, Liu Z H.2017.Tea polyphenol epigallocatechin gallate inhibits Escherichia coli by increasing endogenous oxidative stress[J].Food Chemistry, 217:196-204.
Yuan H B, Meng S H, Wang G Z, Gong Z B, Sun W K, He G Q.2014.Hypoglycemic effect of triterpenoid-rich extracts from Euryale ferox shell on normal and streptozotocin-diabetic mice[J].Pakistan Journal of Pharmaceutical Sciences, 27(4):859-864.
Zhang W N, Su R N, Gong L L, Yang W W, Chen J, Yang R, Wang Y, Pan W J, Lu Y M, Chen Y.2019.Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.seeds[J].Carbohydrate Polymers, 209:363-371.
-
期刊类型引用(4)
1. 王琼，许凤清，邓梦云，任梦婷，王桐生，吴德玲. 芡实壳提取物的抗氧化活性及对口腔溃疡模型大鼠的治疗作用. 南方医科大学学报. 2024(04): 787-794 . 
百度学术
2. 王思锦，尚宏丽，陈佳欣，许家凤. 葡萄皮渣中多酚提取工艺及其抑菌活性研究. 饲料研究. 2023(19): 69-72 . 百度学术
3. 苏可珍，唐月登，王玉牛，胡聪，谢林梦，宋贤良. 响应面法优化软糯口感的粉圆制作配方. 南方农业学报. 2022(02): 516-525 . 本站查看
4. 王纪辉，曾亚军，侯娜，耿阳阳，胡伯凯，刘亚娜，张时馨，杨光. 曲面响应法优化核桃雄花多酚提取工艺及其组成与抗氧化活性分析. 南方农业学报. 2022(10): 2997-3008 . 本站查看
其他类型引用(0)
计量
- 文章访问数: 41
- HTML全文浏览量: 1
- PDF下载量: 1
- 被引次数: 4
下载: