Abstract:
【Objective】To select scientific and rational fungicides for the control of rice bacterial leaf streak
Xanthomonas oryzae pv.
oryzicola(
Xoc), the control effect and applied dose of eight fungicides against rice bacterial leaf streak was evaluated.【Method】Rice bacterial leaf streak susceptible rice cultivar Wushansimiao was material.Wushansimiao at tillering stage was inoculated with dominant virulent strain GDXc1608 of rice bacterial leaf streak isolated from Guangdong area by artificial spraying, and then the disease was controlled by spraying 48 h after inoculation and 7 d after the first application, respectively.The disease index and plant responses to eight fungicides(20% zinc thiazole SC, 50% chloroisobromine cyanuric acid SP, 20% thiodiazole copper SC, 20% bismerthiazol WP, 5% benzisothiazolinone SC, 1.2% xinjunan acetate AS, 3% zhongshengmycin AS and 21.4% cuaminosulfate·copper citrate AS) at the recommended dosage were investigated after the second application.The field efficacy and rice safety of each fungicide were evaluated;and selected four fungicides with better performance and set up three dose gradients in field to carry out pot control experiments in order to determine the best application dosage for the selected fungisides.【Result】The results of field trials showed that among the eight fungicides, 20%zinc thiazole SC had the best control effect on bacterial leaf streak, with the control effect of 71.81%at the dosage of 450.00 mL/ha, followed by 21.4% cuaminosulfate·copper citrate AS, 20% thiodiazole copper SC and 5% benzisothiazolinone SC, the control effects on bacterial leaf streak were 53.50%, 52.00% and 48.23%, respectively.However, the rice plants treated with 21.4% cuaminosulfate·copper citrate AS showed slight phytotoxicity symptoms;the other fungicides showed poor efficacy.The results of pot experiment showed that the control effects of four fungicides on bacterial leaf streak increased with the increase of fungicide dosage, and there were significant differences among different fungicide dosages(
P< 0.05).The control effects of 20% zinc thiazole SC at the dosage of 375.00-525.00 mL/ha were all above 77.00%;the control effect of 20% thiodiazole copper SC was over 62.00% at the dosage of 375.00-525.00 mL/ha.The control effect of 21.4% cuaminosulfate·copper citrate AS was 51.83% at the dosage of 481.50 mL/ha, and the control effect was lower at other test dosages.The control effect of 5% benzisothiazolinone SC was 49.50% and 53.15% at 37.50 and 52.50 mL/ha, respectively.The control effect of 5% benzisothiazolinone SC was low at 22.50 mL/ha.【Conclusion】The control effect of 20% zinc thiazole SC on bacterial leaf streak is better, and it can be used as the first choice for field emergency provention and control of bacterial leaf streak.21.4% cuaminosulfate·copper citrate AS, 20% thiodiazole copper SC and 5% benzisothiazolinone SC also have good control effects on bacterial leaf streak, which can be used alternately with 20% zinc thiazole SC to delay the development of drug resistance.The recommended doses of 20% zinc thiazole SC and 20% thiodiazole copper SC were 375.00-525.00 mL/ha, and the recommended doses of 21.4% cuaminosulfate·copper citrate AS and 5% benzisothiazolinone SC were 481.50 mL/ha and 37.50-52.50 mL/ha, respectively.In order to avoid phytotoxicity, the concentration and spraying time should be strictly controlled when using 21.4% cuaminosulfate·copper citrate AS.