稻飞虱诱导下水稻挥发物分析

Rice volatile components induced by rice planthopper

  • 摘要: 【目的】明确稻飞虱诱导下不同抗性水稻材料挥发物的变化,为新型化学防虫剂研发及水稻抗稻飞虱机理研究提供理论依据。【方法】分别对高抗稻飞虱水稻材料YD1665和高感材料TN1进行稻飞虱取食胁迫,接虫后12 h利用固相微萃取法(SPME)提取褐飞虱取食前后抗感水稻材料的挥发物,并用气相色谱—质谱联用仪(GC-MS)对其化学成分进行鉴定分析。YD1665和TN1未接虫材料分别记为Y0和T0,接虫12 h后材料分别记为Y12和T12。【结果】水稻组成型挥发物鉴定结果表明,2-己烯醛、叶醇和β-紫罗兰酮等挥发物在Y0和T0中的含量均较高;挥发物主要组成成分在2种材料中存在明显差异,2-戊酮和2, 2, 4, 6, 6-五甲基庚烷仅在Y0中检测到,2-乙基呋喃和十六醛仅在T0中检测到。水稻诱导型挥发物鉴定结果表明,Y12以2-戊酮为代表的酮类物质为主,T12以2-己烯醛为代表的醛类物质为主;2-戊酮和2, 2, 4, 6, 6-五甲基庚烷等主要挥发物仅在Y12中检测到,2-乙基呋喃、十五醛、(E, E)-2, 4-己二烯醛和(E, E)-2, 4-庚二烯醛等主要挥发物仅在T12中检测到;2-己烯醛、β-紫罗兰酮为Y12和T12的主要共有挥发物成分。稻飞虱诱导前后水稻挥发物比较分析结果表明,稻飞虱诱导后2种材料的挥发物种类总数均有所增加;Y12的烷类、酮类和醇类相对含量变化最明显,但T12的挥发物种类相对含量变化不明显;2-戊酮、水杨酯甲酯、正己醇、十七烷、叶醇、(E)-2-己烯-1-醇和2, 6-二甲基-环己醇等物质在Y12中变化幅度较大,十五醛、BETA-环柠檬醛、十六醛、(E)-2-己烯-1-醇、叶醇、β-紫罗兰酮和肉豆蔻酸异丙酯等物质在T12中变化幅度较大。【结论】稻飞虱诱导后,水稻体内的水杨酯甲酯、肉豆蔻酸异丙酯、2-戊酮、β-紫罗兰酮、十五醛、十六醛、(E)-2-己烯-1-醇、叶醇、正己醇和2, 6-二甲基-环己醇等物质的变化幅度较大,推测这些挥发物与水稻抗稻飞虱有密切关系。

     

    Abstract: 【Objective】The variation of rice volatiles induced by rice planthopper was determined to provide theoretical basis for for the development of new chemical insecticides and the mechanism of rice resistance to rice planthopper.【Method】YD1665, a rice material with a high resistance to planthopper and TN1, a susceptible material to rice planthopper, were infested by rice planthopper for 0 h or 12 h.They were expressed as Y0, Y12, T0 and T12.The volatile compounds of Y0, Y12, T0 and T12 were extracted before and after rice planthopper feeding by solid-phase microextraction(SPME), and determined by the method of gas chromatography-mass spectroscopy(GC-MS).【Result】The analysis of constitutive volatiles of rice showed that contents of 2-hexenal, leaf alcohol and β-ionone were high in both T0 and Y0.But some components of volatile chemicals were obviously different between them.It was only detected in Y0 for 2-pentanone and 2, 2, 4, 6, 6-pentamethyl-heptane, whilst it was only detected in T0 for 2-ethyl furan and hexadecanal.The analysis of induced volatiles of rice showed that ketones represented by 2-pentanone was the main constituent volatiles of Y12, while aldehydes represented by 2-hexenal was the main constituent volatiles of T12.It was only detected in Y12 for major substances such as 2-pentanone and 2, 2, 4, 6, 6-pentamethyl-heptane, while it was only detected in T12 for major substances such as 2-ethylfuran, pentadecanal, (E, E)-2, 4-hexadienal and(E, E)-2, 4-heptadienal.2-hexenal and β-ionone were the main volatile components in both Y12 and T12.The comparison in volatiles of rice before and after planthopper induction showed that the number of volatile kinds increased in both Y12 and T12 after induction.There were the most obvious changes in content of alkanes, ketones and alcohols in Y12 induced by planthopper.However, there were a little change in T12.There were large variation range in several volatile matters such as 2-pentanone, methyl salicylate, hexanal, heptadecane, leaf alcohol, (E)-2-hexen-1-alcohol and 2, 6-dimethyl-cyclohexanol.There were large variation range in several volatile matters of in Y12, such as pentadecanal, beta-cyclocitral, hexadecanal, (E)-2-hexene-1-alcohol, leaf alcohol, βionone and isopropyl myristate.【Conclusion】There are large variation range in some volatile matters including salicylate methyl ester, isopropyl myricolate, 2-pentanone, β-ionone, pentadecanal, hexadecanal, (E)-2-hexene-1-alcohol, leaf alcohol, hexanal and 2, 6-dimethyl-cyclohexanol after planthopper induction.There might be a close relationship between these substances and rice resistance against planthopper.

     

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