Abstract: ObjectiveThe genetic character and planting performance of the Citrus cultivar Daguo shatangju was ana-lyzed to provide references in order to enrich the variety of Guangxi Shatangju.MethodPloidy analysis and AFLP mo-lecular marker were used to determine the genetics of Daguo shatangju. The botanical characteristics,phenology,yield and fruit character were observed and analyzed.ResultThe ploidy analysis result showed that Daguo shatangju was diploid, the same as Shatangju. The AFLP molecular marker showed that the genetic material of Daguo shatangju was different from Shatangju,Guangmiju,Nanfengmiju,Guiqingju No.1,Qingpiju and Chuntianju. It was a new Citrus gerplasm re-source. Through observation during 2005-2016,it was found that the growth vigor of Daguo shatangju was flourishing. The yield was high and stable. The average fruit mass was 69.0 g. The fruit was oblate with average longitudinal diameter 40.7 mm and average transverse diameter 53.4 mm. The average pericarp thickness was 2.5 mm,edible rate 74.4%,the average total soluble solids content 13.0%,the acid content 0.28 g and vitamin C(Vc)content 33.2 mg/100 gFW. The fruit peel was orange or salmon. The oil cell was little rough and obvious. It was a few-seed cultivar,mature in December in Chongzuo,Guangxi.ConclusionDaguo shatangju is a new Citrus gerplasm resource with genetic specificity,which can be grown extendedly throughout Guangxi Shatangju planting areas due to its high yield,stability,good quality and strong adaptability.