Abstract: ObjectiveMummified aphid was bred in large scale with floating tobacco seedling technology. Then,the best time to collect mummified aphid and the best collecting and packaging method were explored in order to lay the foun-dation for the large-scale production of mummified aphid product.MethodLow-density(25 strains/plate)floating tobac-co seedling was adopted to breed mummified aphid. During the formation period of numerous mummified aphid,the num-ber of mummified aphid and eclosion were counted and investigated every day to analyze the relation between total num-ber of mummified aphid and effective mummified aphid. During the peak formation period of effective mummified aphid, different collecting methods(brush,tube brush and electric mummified aphid brush)and packaging methods(mummified aphid box,mummified aphid card and leaf box of mummified aphid)were adopted. And then,the effects of different co-llecting method on effectiveness of collecting and damage rate to mummified aphid were tested,and the impacts of diffe- rent packaging methods on eclosion of mummified aphid and diffusion of adult bee were analyzed.ResultThe results showed that mummified aphid rapidly formed 11 d post inoculation. On day 18,the number of mummified aphid reached the maximum value(23800.34 aphid/plate),and the eclosion rate also reached 33.49%,the number of effective mummi-fied aphid being only 15830.17 aphid/plate. While 15-17 d post inoculation,the eclosion rate of mummified aphid was be-low 10.00% and the number of effective mummified aphid maintained at more than 20000.00 aphid/plate. By using elec-tric mummified aphid brush,each person could collect 40700.00 aphid each day(8 h),and the eclosion rate of mummi-fied aphid was 86.67%. By using leaf box of mummified aphid and mummified aphid box to package mummified aphid, the eclosion rates were 93.92% and 83.31% respectively and they had no significant difference with the control(P>0.05). By using mummified aphid card to package,the eclosion rate was 66.72% and it had significant difference with the control (P<0.05).ConclusionUnder the condition of 20-30℃,by using floating tobacco seedling(25 strains/plate)to breed mummified aphid,the utilization rate of mummified aphid is high when 15-17 d post inoculation,so it is the best time to collect mummified aphid. By using electric mummified aphid brush,the effectiveness of collecting is high and damage rate is low,so electric mummified aphid brush can better meet the need of large-scale production. Packaging mummified aphid with leaf box of mummified aphid and mummified aphid box had no significant effect on eclosion of mummified aphid and diffusion of adult bee,so they are the suitable packaging methods for mummified aphid.