Abstract: ObjectiveThe effects of three factors including protection time,refrigeration time and acid soaking time on the practical hatching rate of short-term refrigeration and acid treatment,and deferred short-term refrigeration and acid treatment for Liangguang 2 stock were studied to provide references for the adjustment of silkworm egg supply period, the standard formulation on the storage of silkworm eggs and acid stimulus dose in the process of short-term refrigeration and acid treatment of silkworm stock in Guangxi,as well as the improvement of existing short-term refrigeration and acid soaking treatment technology.MethodFour parents of Liangguang 2 stock(Furong×932,932×Furong,Xianghui×7532, 7532×Xianghui)were used as the test materials.Orthogonal test with three levels of three factors including protection time, refrigeration time and acid soaking time was designed to investigate the practical hatching rate of four parents at different levels of factors.The purpose was to find the best combination for increasing hatching rate,and on this basis,parents with fine hatching results were selected to study suitable period of deferred refrigeration.ResultThrough the analysis of orthogonal test,treatment combination of the highest practical incubation rate with desirable factors can be found in diffe-rent refrigerated days for the four parents.In which,Furong×932,932×Furong,Xianghui×7532 and 7532×Xianghui were 48 h-30 d-6 min,48 h-30 d-5 min,48 h-30 d-7 min and 64 h-40 d-6 min respectively.There was not significant difference in practical hatchability between experimental plot and control plot for deferred refrigeration of two parents(Furong×932 and Xianghui×7532)which had sound hatching effects in short-term refrigiration(F