土沉香幼苗对硝普钠—酸铝互作的生理响应

Physiological responses of Aquilaria sinensis seedlings under SNP-AlCl3 interaction stress

  • 摘要: 目的分析土沉香幼苗对硝普钠(SNP)—酸铝(AlCl3)互作的生理响应,为探讨土沉香的耐AlCl3胁迫机理提供理论依据.方法采用完全随机设计,以不同浓度AlCl3胁迫土沉香幼苗并进行SNP-AlCl3互作处理,测定不同处理幼苗的光合色素和可溶性糖含量等生理指标,同时采用隶属函数模糊分析进行综合评价.结果土沉香幼苗叶片的光合色素、可溶性糖和可溶性蛋白含量在0.2 mmol/L AlCl3胁迫下均出现最大值,在0.8 mmol/L AlCl3胁迫下均出现最小值;丙二醛(MDA)含量在0.2 mmol/L AlCl3胁迫下最低,在0.8 mmol/L AlCl3胁迫下最高,而超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性相反.添加SNP后,土沉香幼苗叶片的叶绿素a、叶绿素b、类胡萝卜素、可溶性糖和可溶性蛋白含量及SOD、POD和CAT活性等均明显高于未添加SNP处理.相关性分析结果表明,叶绿素总量、叶绿素a、叶绿素b、类胡萝卜素、可溶性糖和可溶性蛋白含量及SOD、POD和CAT活性等指标间均呈极显著正相关(P<0.01).结论低浓度(0.2 mmol/L)AlCl3胁迫可促进土沉香幼苗生长,外源添加SNP对高浓度(0.8 mmol/L)AlCl3胁迫土沉香幼苗产生的毒害具有一定缓解作用,可在土沉香幼苗培育及抗性研究等相关领域推广应用.

     

    Abstract: ObjectiveThe physiological response of Aquilaria sinensis seedlings to sodium nitroprusside(SNP)-acid aluminum(AlCl3)interaction stress were analyzed to provide theoretical basis for further studying the resistance mecha-nism of A. sinensis against AlCl3stress.MethodA completely random design was applied,A. sinensis seedlings were stressed by different concentrations of AlCl3and treated with SNP-AlCl3interaction.Physiological indexes such as photo-synthetic pigment and soluble sugar content of A.sinensis under different treatment were detected.Fuzzy analysis method of membership function was used for comprehensive evaluation.ResultPhotosynthetic pigments,soluble sugar content and soluble protein content in the seedling leaves of A.sinensis reached the maximum under 0.2 mmol/L AlCl3stress,and were the minimum under 0.8 mmol/L AlCl3stress. Malondialdehyde(MDA)content were the lowest when under 0.2 mmol/L AlCl3stress,and were the highest when under 0.8 mmol/L AlCl3stress,while activities of superoxide dismutase (SOD),peroxidase(POD)and catalase(CAT)were on the contrary.After adding SNP the physiological indexes in A.si-nensis seedling leaves such as contents of chlorophyll a,chlorophyll b,carotenoid,soluble sugar and soluble protein activi-ties of SOD,POD,CAT were largely higher than those without SNP.Correlation analysis indicated that,for the indexes in-cluding contents of total chlorophyll,chlorophyll a,chlorophyll b,carotenoid,soluble sugar and soluble protein,activities of SOD,POD and CAT there were extremely significant positive correlation between one and another(P<0.01).Conclu-sionApplying low concentration(less than 0.2 mmol/L)AlCl3solution promotes A.sinensis seedlings growth.Exogenous addition of SNP has obvious mitigative effects on the toxicity caused by high concentration(0.8 mmol/L)AlCl3stress in A. sinensis seedlings,which can be applied in the related field such as cultivation and resistance research of A.sinensis seed-lings.

     

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