Abstract: ObjectiveProcess conditions of ultrasonic-assisted extraction for absolute from Elsholtzia rugulosa Hemsl.were optimized and its application effects in cigarette were analyzed to provide technical reference for further ap-plication of E.rugulosa in cigarettes.MethodWith E.rugulosa as raw materials,ultrasonic-assisted ethanol extraction conditions were optimized by single factor test and orthogonal experiment.Component analysis was also conducted.After E. rugulosa absolute was added into cigarettes,the optimal adding amount was determined through sensory evaluation.ResultThe optimal conditions for ultrasonic-assisted extraction of E. rugulosa absolute were as follows: extracting at 50℃for 40 min with a solid-liquid ratio of 1:9 under an ultrasonic power of 500 W.The order of influencing factors was:extraction temperature>ultrasonic power>solid-liquid ratio>extraction time. Identified by gas chromatograph-mass spec-trometer(GC/MS),40 components were identified from E.rugulosa absolute,such as dehydroelsholtzia ketone(28.641%), 3,3-dimethylacrylic acid(9.080%),5-methyl-2-acetylfuran(4.790%),linalool(4.224%),3-methyl-2(5H)-furanone(3.642%), linalool oxide(3.079%),elsholtzia ketone(1.436%),neophytadiene(1.031%),etc.The result showed that the optimum amount of E.rugulosa absolute in cigarette was 3 mg/kg,which could enrich and coordinate the cigarette aroma,improve aroma quality and taste,make the smoke soft and fine,reduce the irritating sense and remove impurities.ConclusionE.rugulosa absolute extracted by optimized ultrasonic-assisted method can improve smoking quality of cigarette.Hence it is available for flavoring and quality improvement of cigarette.