Abstract: ObjectiveThe objective of this study was to screen the optimal condition for in vitro rapid propagation of Styrax faberi Perk. to provide the reference for S. faberi planting promotion and eco-environmental modification.MethodThe seeds of S.faberi with or without endosperm were used as explants,MS as the basic medium and different concentrations of GA3were used for aseptic germination of seeds.Stem of aseptically germinated seeds were used as ex-plants,MS was used as the basic medium,the optimal combinations of 6-BA,NAA and TDZ were screened for prolifera-tion of clustered shoots of S.faberi by orthogonal experiment.Seedlings obtained by propagation of clustered shoots as ex-plants,MS or 1/2MS as the basic medium,different concentrations of IBA were added,direct or indirect rooting method were used to screen optimal basic medium,IBA concentration and rooting method for the rooting of S.faberi.ResultThe results showed that cotyledon of S.faberi seeds with endosperm on media with different concentrations of GA3were browning when germinated after 15 d and then died.Seeds without endosperm could germinate even on the medium with-out any hormone.The optimal medium for seed germination without endosperm was MS+2.0 mg/L GA3,and the germina-tion rate was up to 97.5%.At the stage of subculture multiplication,the optimal medium of clustered shoots proli-feration was MS+0.5 mg/L 6-BA+0.05 mg/L NAA,where the proliferation coefficient was 5.7.At rooting stage,when the stem segments with two sections were soaked in IBA solution of 200.0 mg/L for 15 min and then transferred to 1/2MS blank medium,the rooting rate was the highest,reaching 93.3%,and the average number of roots was 7.1.ConclusionThe seed germination rate of S.faberi is the highest in MS+2.0 mg/L GA3medium and the proliferation coefficient of S.faberi clustered shoots is the highest in MS+0.5 mg/L 6-BA+0.05 mg/L NAA medium.Indirect rooting method can be used to ob-tain S.faberi tissue culture seedlings with high rooting efficiency and large-scale production.