Abstract: ObjectiveGenotype and gene-environment interaction of tested varieties in Guangxi fresh-eaten maize two seasons per year regional trial were evaluated comprehensively to provide reference for maize regional trial and breeding.MethodGGE biplot analysis was conducted on the fresh ear yield of Guangxi fresh-eaten wax maize regional trial in spring and autumn of 2016 by GGE-biplot software. Fertility,stability,adapted region of tested maize varieties and the representativeness and discrimination of each trial site were assessed.ResultComprehensive analysis results of tested maize varieties and environment indicated that there was positive correlation among most trial sites. There was close posi-tive correlation between Beihai and Yulin test sites in spring and Beihai and Liuzhou test sites in autumn,and they had similar influence on yield rank. According to the analysis of optimal environment for tested maize varieties,the seven test sites in spring could be divided into four ecological districts and seven test sites in autumn could be divided into three ecological districts,which presented the adaptability of the tested varieties intuitively. The analysis on fertility and stability of tested varieties showed that Gui W1518 in spring and Baixiangnuo 1601 in autumn had sound fertility and stability performances in the corresponding seasons. Analysis on environment representativeness and discrimination of trial sites in-dicated that Liuzhou and Nanning were ideal trial sites in spring,while in autumn Yulin and Hechi were optimal ones.ConclusionGGE biplot can show directly the fertility,stability,adaptability of tested varieties and discrimination,repre-sentativeness,ecological types of trial sites,which is of importance for maize regional trial and maize breeding.