山黄皮果实和叶片挥发油成分分析

Chemical constituents of essential oils from fruit and leaf of Clausena anisum-olena (Blauco) Merr.

  • 摘要: 目的考察山黄皮桂研15号果实(含采肉和果皮)和叶片挥发油化学成分差异,以挖掘山黄皮桂研15号的利用价值,为山黄皮资源的开发利用提供科学依据.方法采用水蒸汽蒸馏法提取山黄皮果实和叶片挥发油,通过气相色谱—质谱联用仪(GC-MS)对山黄皮不同部位挥发油化学成分进行分析;通过NIST标准质谱图库分别鉴定各化学组分;经面积归一化法测定山黄皮不同部位挥发油中化学成分的相对含量.结果从山黄皮桂研15号果实和叶片中共鉴定出55种化合物,其中萜烯类28种、醇类4种、醛类6种、酮类2种、酯类4种、碳氢化合物类6种、醚类5种.从山黄皮果肉、果皮及叶片挥发油中分别鉴定出20、31和34种化合物,其相对含量占各自挥发油总量的76.97%、95.11%和83.85%.山黄皮果肉、果皮及叶片挥发油的主要成分分别为单萜类化合物β-蒎烯、月桂烯和萜品油烯.因此,山黄皮桂研15号果实和叶片挥发油化学成分以萜烯类化合物(主要是单萜类化合物)和醚类化合物为主.结论山黄皮桂研15号果实和叶片挥发油化学成分成分种类和含量存在明显差异,且有独特的挥发油成分.综合成本因素,山黄皮桂研15号叶片挥发油具有更大的开发潜力.

     

    Abstract: Objective The differences between the chemical constituents of essential oils from fruit (including pulp and peel) and leaf of C.anisum-olena (Blauco) Merr,were investigated to explore the utilization value of Guiyan No.15 and provide reference for the development and utilization of it.MethodThe essential oils were extracted by steam distillation from fruit and leaf of C.anisum-olena.The chemical constituents of essential oils from different parts were analyzed by gas chromatograph-mass spectrometer (GC-MS) method,and identified by Mass Spectral Standard Database NIST.The relative content of chemical constituents of essential oils from differnt parts were determined by area normalization method.Result Fitty-five compounds in fruit and leaf of C.anisum-olena were identified,including twenty-eight terpenes,four alcohols,six aldehydes,two ketones,four esters,six hydrocarbons and five ethers.Twenty,thirty-one and thirty-four compounds were identified from fruit pulp,peel and leaf of C.anisum-olena respectively.The relative contents of identified compounds accounted for 76.97%,95.11% and 83.85% of total essential oils in respective parts.The main constituents in fruit pulp,peel and leaf of C.anisum-olena were monoterpenes,beta-pinene in pulp,myrcene in peel and terpinolene in leaf specificly.Therefore,terpene compounds (mainly monoterpenes) and ethers were the major chemical constituents in the essential oils from fruit and leaf of C.anisum-olena.ConclusionThere are significant differences in chemical constituents and relative contents between essential oils from fruit and leaf of C.anisum-olena,and unique essential oils are indentified.Considering cost,essential oils from leaf of C.anisum-olena have greater potential in development.

     

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