Abstract: ObjectiveThe effects of copper(Cu2+) stress on antioxidant and immune parameters in serum of Macro-brachium rosenbergii were studied to provide the reference for aquaculture environment regulation. MethodTwo Cu2+stress concentrations, 0(control group) and 100 μg/L(stress group) were designed, after 0, 3, 6, 12, 24 and 48 h of stress, serum was separated. The total anti-oxidative capacity(T-AOC), activities of catalase(CAT), malondialdehyde(MDA), acid phosphates(ACP) and alkaline phosphatase(AKP) in serum were detected. ResultT-AOC in serum significantly increased after Cu2+ stress for 3-12 h (P<0.05, the same below), and reached the peak at 12 h, then decreased significantly at 24 h and 48 h of stress. Compared with control group, the activity of CAT in stress group significantly increased at 3 h of stress, and then decreased significantly at 6 h, but increased again and reached the peak at 12 h. Thereafter, CAT activity decreased gradually, and was significantly lower than that of control group at 48 h. MDA content in stress group rose then slightly declined during the whole stress procedure, and was significantly higher than that of control group at all time points. ACP activity significantly increased after 6 and 12 h of stress, and then recovered to the original level at 24 and 48 h. There is no significant change for AKP activity after stress for 3-12 h(P>0.05), but it significantly decreased after 24 and 48 h.ConclusionCu2+ stress lead to lipid peroxidation in M. rosenbergii, and antioxidant system and immune system are in-duced to resist Cu2+ stress. Moreover, antioxidant and immune parameters variation in M. rosenbergii display time-depen-dent response. T-AOC, CAT activity and MDA content are sensitive to Cu2+ stress, which are significantly induced after short time stress. Therefore, they can be used as the potential biological indicators of Cu2+ pollution in aquaculture.