Abstract: ObjectiveIn order to provide theory basis for integrated control and disease-resistant breeding against rice ragged stunt disease(RRSD),this paper determined the resistance level of Guangxi major rice varieties to RRSD. MethodUsing the method of artificial inoculation identification at seedling stage, the resistance levels of 45 Guangxi major rice varieties to RRSD were determined based on their symptoms and RT-PCR detection results. ResultAmong the 45 rice varieties, there was no resistant variety against RRSD. The results showed that fourty-one varieties were highly susceptible to RRSD, accounting for 91.11%; four varieties were moderately susceptible, accounting for 8.89%. ConclusionThe current major rice varieties in Guangxi lacks resistance against RRSD.