Abstract: ObjectiveSelenium enriched Moldova grape was researched based on polynomial regression analysis , so as to provide reference for planting selenium enriched grapes in Guangxi selenium enriched field without adding selenium substance. MethodMoldova grape was used as the material in experiment. With ternary quadratic saturation D-optimum design,limestone powder(x1),AM fungal inoculum(x2), nutrient regulators(x3) as dependent variables, selenium content in grape as independent variable(y), 11 treatments(treatment 1-11) were set to conduct regression analysis. Through the ex-periment, effects of experiment factors on selenium content in Moldova grape were studied, in order to determine applica-tion rates of allogenic materials for selenium enriched grapes. ResultIn all treatments applying limestone powder, AM fungal inoculum and nutrient regulators(except treatment 1), selenium contents in Moldova grape increased 1.1 to 1.8 times compared with CK, the maximum increasement reached 87.2%. Single factor effect results showed that selenium content in grapes was the highest when applying 2400.00 kg/ha limestone powder, 69.00 kg/ha AM fungal inoculum and 39.98 kg/ha nutrient regulators, and the selenium content in Moldova grape was 11.56 μg/kg. Interaction effects analysis indicated that effects rank of two factors interaction on Moldova grape were as follows:x1x3>x1x2>x2x3. ConclusionApplying 2400.00 kg/ha limestone powder, 69.00 kg/ha AM fungal inoculum and 39.98 kg/ha nutrient regulators to Moldova grape plants, the fruits can reach selenium enriched level. Therefore, application of limestone powder, AM fungal inoculum and nutrient regulators can be utilized as a new technique for planting selenium enriched grapes.